CSIR UNIT 13 Flowchart
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CSIR UNIT 13 Flowchart & Mind Maps Download

Biotecnika is a well-known name in the field of life sciences exam preparation. We have been providing high-quality coaching and study materials to students for several years. Biotecnika’s CSIR NET Life Science Exam Preparation tools have received high acclaim from students and have helped many crack the CSIR NET with high JRF and LS ranks. Yet again, we are here to make your CSIR NET preparation easy & smart with the use of Flowcharts, Mind Maps & Notes for CSIR NET UNIT 13: Methods in Biology.

CSIR NET Unit 13: Methods in Biology is a vast unit that covers various techniques and their applications in experimental research. This unit is known to test the true research abilities of candidates, and it is expected that extremely experimental questions will be asked. Although scoring cannot be guaranteed from the first three subunits, direct questions are asked from the next four subunits. Thus, it is essential to prepare well and focus on the following important topics to have an edge.

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Some important topics of CSIR NET Life Science UNIT 13: Methods in Biology are

listed below:

  • Recombinant DNA technology is a crucial topic, which includes RNA, DNA, and proteins isolation, separation, and analytical techniques such as 1D and 2D gel electrophoresis, isoelectric focusing gels, cloning, recombinant protein expression using various bacterial, animal, and plant vectors (cosmid, BAC, and YAC vectors), genomic and cDNA libraries, in vitro mutagenesis and deletion techniques, gene knock-out, DNA and protein sequencing, gene expression analysis at RNA and protein level, microarrays, RFLP, RAPD, and AFLP techniques.

Sample of CSIR NET UNIT 13 Mind Map Downloads

CSIR UNIT 13 Flowchart

  • Immunological techniques such as ELISA, RIA, western blot, immunoprecipitation, flow cytometry, immunofluorescence microscopy, FISH, and GISH are also important topics to study. Biophysical methods such as UV/visible, fluorescence spectroscopy, circular dichroism, NMR, ESR spectroscopy, X-ray diffraction, NMR, and different types of mass spectrometry should also be understood.
  • Statistical methods such as probability distributions such as Binomial, Poisson, and normal, T-test, and X2 test are crucial for this unit. Radiolabeling techniques and various types of radioisotopes used in biology and their properties focus on their detection, measurement, and incorporation are also important topics.
  • Microscopic techniques, numerical resolving powers of different microscopes, SEM, TEM, fixation, and staining techniques, freeze-etch, and freeze-fracture methods for EM, and electrophysiological methods such as single neuron recording, patch-clamp recording, ECG, Brain activity recording, PET, MRI, fMRI, CAT should also be studied.

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CSIR NET UNIT 13 Reference Books:

Reference books such as Principles of Gene Manipulation and Genomics by Sandy B. Primrose, Richard Twyman, Gene Cloning and DNA Analysis: An Introduction by T. A. Brown, Molecular Cloning: A Laboratory Manual by Joseph Sambrook, David Russell, Fundamentals of Biostatistics by V.B. Rastogi, and Principles and Techniques of Biochemistry and Molecular Biology by Wilson/Walker can be helpful for preparation.

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