AIIMS Delhi Biotech, Biochem, Biophysics & Bioinformatics JRF Job – Applications Invited

Question 1: Can you provide examples of your experience in cloning techniques and how they relate to the restoration of p53 function in breast cancer mutants? Answer: Certainly. I have hands-on experience in molecular cloning, including gene amplification, vector construction, and transformation. In the context of the project, I’ve successfully cloned p53 oligomerization domain mutants into expression vectors, which is a crucial step in screening therapeutic activator molecules.

Question 2: Protein purification and crystallization are essential aspects of this project. Could you explain your familiarity with these techniques and their relevance to studying mutant p53 proteins? Answer: Absolutely. I have performed protein purification using techniques like affinity chromatography and gel filtration, isolating proteins for downstream studies. Regarding crystallization, I’ve successfully crystallized proteins and conducted X-ray diffraction analysis to determine their structures. In this project, these techniques will help us study the interactions and conformational changes of p53 mutants.

Question 3: The project involves screening for therapeutic p53 activator molecules. How have you utilized your biophysical skills to evaluate molecular interactions in your previous work? Answer: In a previous project, I used techniques like surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) to study protein-ligand interactions. By quantifying binding affinities and thermodynamic parameters, I gained insights into molecular recognition. Similar biophysical methods can be employed here to assess the binding of activator molecules to p53 mutants.

Question 4: Could you discuss the role of Bioinformatics in this project and provide an example of how you’ve utilized it in your research? Answer: Bioinformatics plays a pivotal role in analyzing complex biological data. For instance, in a prior study, I employed computational tools to predict protein-protein interactions and modeled the 3D structure of a protein complex. In this project, Bioinformatics can aid in virtual screening of potential activators and predicting their binding modes to p53 mutants.

Question 5: The project has a duration of six months. How do you plan to manage your time effectively to achieve the project’s objectives within this timeframe? Answer: Time management will be crucial. I’ll start by breaking down the project into stages, each with specific goals and timelines. Regular progress assessments and effective communication within the team will ensure that we stay on track. If needed, I’ll adapt the plan based on interim results. By efficiently allocating resources and coordinating tasks, I’m confident in meeting the project’s objectives in the given timeframe.

(Note: The answers provided are samples and can be tailored based on the interviewee’s actual experiences and knowledge.)