Syngene Research Associate Job For MSc, MTech Life Sciences, Biotech, Cell Biology
Syngene Research Associate Job For MSc, MTech Life Sciences, Biotech, Cell Biology. MSc, MTech Biotechnology/ Cell Biology, Life Sciences Job in Syngene, Bangalore. Syngene is hiring Biological Sciences candidates for the Research Associate / Senior Research Associate job.
The five possible interview questions that can be asked in the technical round for the Research Associate / Senior Research Associate position, along with their answers are posted below:
JOB DESCRIPTION
Designation: Research Associate / Senior Research Associate
Req ID: 54450
Job Location: Bangalore
Department: BBRC-Translational Medicine (TM) – Protein Science
About Syngene
Incorporated in 1993, Syngene International Ltd. is an innovation-focused global discovery, development and manufacturing organization providing integrated scientific services to the pharmaceutical, biotechnology, nutrition, animal health, consumer goods and specialty chemical industries around the world. Syngene’ s clientele includes world leaders, such as Bristol-Myers Squibb, Baxter, Amgen, GSK, Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240-strong team of scientists who work with clients from around the world to solve their scientific problems, improve R&D productivity, speed up time-to-market and lower the cost of innovation.
Job Purpose:
Be a part of
protein science team and perform recombinant DNA techniques.Key Responsibilities:
- Design and perform recombinant DNA techniques like PCR, RT-PCR,
- Cloning techniques like Ligation based cloning, recombination based cloning, shRNA cloning, Sub cloning
- Site directed mutagenesis
- Sanger sequence data analysis
- Genomic DNA/RNA isolation techniques
- Knowledge of software like Vector NTI/Geneious/ SnapGene
- Diligent documentation skills and experience writing detailed E-Lab/Lab Notebooks
Educational Qualification and Experience:
M.Tech / M.Sc or similar qualifications in Biotechnology/ Cell Biology/ Biomedical Sciences/ Life Sciences with 1-2 years of research experience.
Technical/Functional Skills:
- Design and perform recombinant DNA techniques like PCR, RT-PCR,
- Cloning techniques like Ligation based cloning, recombination based cloning, shRNA cloning, Sub cloning
- Site directed mutagenesis
- Sanger sequence data analysis
- Genomic DNA/RNA isolation techniques
- Knowledge of software like Vector NTI/Geneious/ SnapGene
- Diligent documentation skills and experience writing detailed E-Lab/Lab Notebooks
DESIRABLE
- Reagent inventory maintenance
- Experience with liquid handlers
- High throughput cloning techniques
Behavioural Skills:
- A proactive team player
- Ensure completion of experiments and generate accurate and reproducible data from experiments.
- Ability to work independently, prioritize tasks and work on multiple projects simultaneously with; comfortable working in a dynamic environment with changing requirements.
- Good oral and written communication and presentation skills to work in a multi-disciplinary team.
Equal Opportunity Employer:
It is the policy of Syngene to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by applicable legislation or local law. In addition, Syngene will provide reasonable accommodations for qualified individuals with disabilities.
Here are five possible interview questions that can be asked in the technical round for the Research Associate / Senior Research Associate position, along with their answers:
- Can you provide examples of your experience in performing recombinant DNA techniques such as PCR, RT-PCR, and cloning techniques?
- Answer: Certainly! In my previous research experience, I have extensively worked with PCR and RT-PCR for amplifying and analyzing specific DNA sequences. I have also utilized cloning techniques like ligation-based cloning and recombination-based cloning to construct expression vectors for protein production. I can share specific projects where I successfully employed these techniques and achieved the desired results.
- How familiar are you with site-directed mutagenesis? Can you explain the process and any challenges you encountered while performing it?
- Answer: I have hands-on experience with site-directed mutagenesis, which involves introducing specific mutations in DNA sequences. The process typically involves primer design, amplification, and subsequent transformation to incorporate the desired mutation. During my previous research, I encountered challenges such as optimizing primer design and troubleshooting low transformation efficiency. I can discuss how I overcame these challenges and achieved successful mutagenesis.
- Have you worked with Sanger sequencing data analysis? How do you ensure accurate and reliable sequence analysis?
- Answer: Yes, I have worked extensively with Sanger sequencing data analysis. To ensure accurate and reliable analysis, I follow a rigorous workflow that includes quality assessment, trimming of low-quality bases, alignment to reference sequences, and variant calling. I also cross-check results with complementary software tools to validate the findings. Attention to detail and thorough documentation of analysis steps are crucial for reliable sequence analysis.
- Can you explain your experience with genomic DNA/RNA isolation techniques? Which methods have you used, and how do you ensure high-quality and intact nucleic acid extraction?
- Answer: In my previous research, I have employed various genomic DNA/RNA isolation techniques such as phenol-chloroform extraction, column-based purification, and magnetic bead-based methods. I prioritize the use of validated protocols and optimize extraction conditions to ensure high-quality and intact nucleic acid isolation. Quality assessment through spectrophotometric analysis and gel electrophoresis is performed to verify the integrity and purity of the extracted nucleic acids.
- Have you used software tools like Vector NTI, Geneious, or SnapGene for molecular biology analysis? Can you describe your proficiency in utilizing these tools?
- Answer: Yes, I am proficient in using Vector NTI, Geneious, and SnapGene for molecular biology analysis. These software tools are crucial for primer design, sequence alignment, plasmid mapping, and construct visualization. I have utilized these tools to design primers, analyze DNA sequences, construct plasmid maps, and annotate genetic elements. I am comfortable navigating through the interfaces of these tools and utilizing their functionalities effectively.
Please note that these are sample interview questions, and the actual questions asked in the interview may vary depending on the interviewer and the specific requirements of the job position.
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