PGIMER Bioinformatics & Cell Biology JRF Job – Applications Invited

Possible interview questions for the technical round of the Junior Research Fellow (JRF) position in the “Role of miR193a……… in patients with Psoriasis” project:

1. Can you explain the significance of miR193a in the context of psoriasis and its potential role in disease pathology? Answer: miR193a is a specific microRNA molecule that has been implicated in various biological processes, including the development and progression of psoriasis. It is known to regulate the expression of target genes involved in inflammatory responses, cell proliferation, and immune modulation. Understanding the role of miR193a in psoriasis can provide insights into the underlying mechanisms of the disease and potential therapeutic targets.
2. Describe your experience and proficiency in bioinformatics analysis related to studying microRNA. Answer: I have practical experience in performing bioinformatics analysis related to microRNA research. I am familiar with databases and tools used for miRNA target prediction, sequence analysis, and functional annotation. I have worked with software such as miRBase, TargetScan, and miRanda for miRNA target identification. Additionally, I am skilled in analyzing high-throughput sequencing data to identify differentially expressed miRNAs and potential regulatory networks.
3. Can you discuss your expertise in conducting experiments involving animal cell biology, specifically related to psoriasis research? Answer: I have gained experience in working with animal cell cultures during my academic and research pursuits. I am proficient in cell culture techniques, including cell line maintenance, transfection, and treatment protocols. I have worked with animal cell models to investigate cellular processes relevant to psoriasis, such as keratinocyte proliferation, apoptosis, and inflammatory responses. I am well-versed in using techniques like immunofluorescence, Western blotting, and qPCR for data generation and analysis.
4. How would you design experiments to study the role of miR193a in patients with psoriasis? Answer: Designing experiments to investigate the role of miR193a in psoriasis would involve multiple steps. Firstly, I would collect patient samples and isolate RNA to analyze miR193a expression levels. I would perform functional studies by modulating miR193a expression in relevant cell models using techniques like transfection or knockdown approaches. Subsequently, I would evaluate the impact of altered miR193a expression on key cellular processes associated with psoriasis, such as inflammation or keratinocyte proliferation, using appropriate assays and readouts.
5. Can you discuss any previous research projects or coursework that have equipped you with the necessary skills and knowledge for this JRF position? Answer: Throughout my academic journey, I have undertaken research projects and completed relevant coursework that align with the requirements of this position. For instance, I conducted a project investigating the role of microRNAs in skin diseases, where I gained hands-on experience in miRNA profiling, target prediction, and functional characterization. I have also completed courses in bioinformatics, cell biology, and molecular biology, which have provided me with a solid foundation for studying miRNAs and their roles in disease.

Note: The answers provided here are general guidelines. It’s advisable to tailor your responses based on your own experience, qualifications, and familiarity with the specific project and job requirements.