New ways for developing biomarkers for diseases – by DBT-InSTEM scientists
The capability to evaluate proteomic changes in the blood dynamically has vital ramifications to the exploration as well as tracking of biomarkers for a variety of infectious metabolic, and myeloid diseases. The biomarkers can vary from the detection of viral/bacterial peptides to validate whether a certain biochemical pathway has been involved by a given medication as a part of treatment effectiveness surveillance. But, till now it has been cumbersome to identify newly synthesized proteins from the whole blood-derived from typical blood collection approaches.
The faculty from the Centre for Neurodevelopmental Synaptopathies collaboratively with the researchers from New York University and Mass Spectrometry Facility at the Bangalore Life Science Cluster have actually developed an approach to incubate, label, and then find detect newly synthesized proteins in freshly collected blood via mass spectrometry.
The team was able to determine proteins from erythrocytes, lymphocytes, and also platelets in samples – Evaluating the evidence of concept in mice as well as rat blood. The proteomic work performed in this study has embraced cutting edge peptide identification algorithms to improve peptide detection.
This study provides proof of principle evidence for what might be rapidly
deployable approach to label newly synthesized proteins in the freshly collected blood samples to detect dynamic protein shifts as well as develop novel biomarkers for a variety of diseases. The outcomes of the study have been released in the journal of American Chemical Society’s Journal of Proteome Research entitled ‘Optimization of protocols for the discovery of de novo protein synthesis in whole blood samples through azide-alkyne cycloaddition.’
inStem – Institute for Stem Cell Science and Regenerative Medicine is dedicated to the research of stem cells as well as regenerative biology. Research at inStem addresses complicated issues in areas of cell-differentiation and also tissue regeneration using human pluripotent cells, and examines scientific indications of diseases that can possibly be treated by stem cells.
Author: Sruthi S