COVID-19 Research Consortium

BIRAC Invites Applications From All Bio-Professionals To Work On COVID-19 Research

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COVID-19 Research Consortium - BIRAC Call For Proposals. In view of the uncertainty with regards to the COVID-19 pandemic, there is a dire need to...

Biotecnika Times – Newsletter 20.03.2020 – CMR JRF 2020 Exam, CUCET 2020 Notification, Govt

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Biotecnika Times - CMR JRF 2020 Exam, CUCET 2020 Notification, Govt ICMR JRF 2020 Exam Notification – Eligibility, Application Details & Deadline ICMR JRF 2020 Exam...
MSc Admission Notification 2020

MSc Plant Biotech Admission Notification 2020 at TERI School of Advanced Studies (TERI SAS)

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MSc Admission Notification 2020 at (TERI SAS) The program has been formulated with the objective of advancing education and research in the area of biotechnology...

ICMR JRF 2020 Exam Notification – Eligibility, Revised Application Details & Deadline

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ICMR JRF 2020 Exam Notification - Eligibility, Application Details & Deadline ICMR JRF 2020 Exam Notification - Eligibility, Application Details & Deadline. Interested and eligible...
Bill Gates Warned Us About Coronavirus

How We Ignored Bill Gate’s Warning On An Epidemic Five Years Back

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Bill Gates Warned Us About Coronavirus - But We Chose To Ignore Bill Gates knew years ago that an epidemic like coronavirus is coming and...
Facts of Coronavirus

Coronavirus Fact Check – Can Face Masks Prevent COVID-19?

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Facts of Coronavirus Covid-19 The reality regarding exactly how you can get infected by coronavirus COVID-19, Who is most susceptible and How to avoid...
New Hand Sanitizer By IHBT scientists

New Hand Sanitizers Developed By IHBT Scientists To Prevent Coronavirus Infection

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New Hand Sanitizer By IHBT scientists Amidst reports of preventive measures against coronavirus and several counterfeit materials being sold in the market, the demand for...
CUCET 2020 Notification - Central Universities Common Entrance Test

CUCET 2020 Notification – Central Universities Common Entrance Test

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CUCET 2020 Notification - Central Universities Common Entrance Test CUCET 2020 Notification - Central Universities Common Entrance Test. The application portal is now open as...

Biotecnika Times – Newsletter 19.03.2020 – Cancer Center CNCI Job, NCBS Graduate Trainee

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Biotecnika Times - Cancer Center CNCI Job, NCBS Graduate Trainee Govt Regional Cancer Center, CNCI Life Science Research Fellow Recruitment Regional Cancer Center Jobs – Life...

Is Coronavirus A Man Made Bioweapon?

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Coronavirus Laboratory Created Or Not - Scientists Provide Evidence According to findings published today in Nature Medicine, the novel coronavirus pandemic that emerged in the Chinese...
Summer Training Program 2020

Summer Training Program 2020 at Dept of Bioengineering, NIT Agartala

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Summer Training Program 2020 at Dept of Bioengineering, NIT Agartala Summer Training Program 2020 at Dept of Bioengineering, NIT Agartala notification is out. Summer Training...
Editing Multiple Genome Fragments At A Time Scientists can now edit multiple sites in the genome at the same time to learn how different DNA stretches co-operate in health and disease. CRISPR-based DNA editing has revolutionized the study of the human genome by allowing precise deletion of any human gene to glean insights into its function. But one feature remained challenging—the ability to simultaneously remove multiple genes or gene fragments in the same cell. Yet this type of genome surgery is key for scientists to understand how different parts of the genome work together in the contexts of both normal physiology and disease. Now such a tool exists thanks to the teams of Benjamin Blencowe and Jason Moffat, both professors of molecular genetics at the Donnelly Centre for Cellular and Biomolecular Research. Dubbed 'CHyMErA', for Cas Hybrid for Multiplexed Editing and Screening Applications, the method can be applied to any type of mammalian cell to systematically target the DNA at multiple positions at the same time, as described in a study published in the journal Nature Biotechnology. Often described as genome scissors, CRISPR works by sending a DNA-cutting enzyme to desired sites in the genome via guide RNA molecules, engineered to adhere to the target site. The most widely used DNA-cutting enzyme is Cas9. - Editing Multiple Genome Fragments At A Time. Since Cas9 first came to light, other Cas enzymes with distinct properties have been identified by scientists seeking to improve and expand the applications of the technology. Unlike the CRISPR-Cas9 technology, CHyMErA combines two different DNA-cutting enzymes, Cas9 and Cas12a, to allow more versatile applications. Cas12a is an enzyme that can be used to generate multiple guide RNA molecules in the same cell, which is key for simultaneous DNA editing. Thomas Gonatopoulos-Pournatzis, a research associate in Blencowe's group, had spent several years trying to develop combinatorial gene editing by testing Cas9 and Cas12a enzymes on their own. He then had the idea to combine these enzymes to generate the CHyMErA system. "We had been trying a number of approaches to induce genetic fragment deletions and nothing worked as well as CHyMErA," he says. "I was thrilled when together with Shaghayegh Farhangmehr, a Ph.D. student in the Blencowe lab, we saw the first evidence that CHyMErA was successful in deleting gene segments. We obtained these results on Boxing Day and it was the best Christmas present I could have wished for." The next step was to harness CHyMErA in large-scale screens to systematically analyze how genes act together, as well as the functions of individual parts of genes. Blencowe's team, which studies the regulation and function of gene segments known as exons, approached Moffat, whose group had developed extensive experience with CRISPR technology. "With CHyMErA, you can use the best of the two enzymes," says Michael Aregger, a research associate in the Moffat lab, who played a key role in developing the screen-based applications of CHyMErA. "Cas9 has been improved by the community to have a very high editing efficiency, whereas Cas12a allows multiplexing of guide RNAs and therefore provides a lot more flexibility in finding sites in the genome that we can cut." In one application of CHyMErA, the researchers targeted pairs of genes known as paralogs, which have a similar DNA code but remain poorly studied because they were difficult to research. Because paralogs arose by duplication of an ancestral gene, it had been assumed they would largely have similar roles. But their function could not be revealed by the existing single-gene targeting methods typically employed in genetic screens, mostly because the other paralog would compensate for the one that's missing. "With CHyMErA, we can take out both paralogs in pairs to see if that ancestral function is important for the cell to survive," says Kevin Brown, a senior research associate in the Moffat lab and co-lead author on the study along with Aregger and Gonatopoulos-Pournatzis. "We are able to now interrogate a class of genes that were previously missed." After knocking out ~700 paralog pairs, almost all that exist in the human genome, the analysis confirmed that many of these gene pairs do indeed perform similar roles in cell survival, whereas others have distinct functions. Another feature of CHyMErA is that both Cas9 and Cas12a can be deployed to nearby genome sites to cut out gene fragments such as exons. This allowed the team to individually delete thousands of exons that have been linked to cancer and brain function but were not amenable to targeting with Cas9 alone. Exons are variably included in genes' transcripts and can modify the function of the encoded proteins, although how individual exons contribute to cellular processes remains largely unknown. Out of 2,000 exons analyzed by CHyMErA, over 100 were found to be critical for cell survival, enabling future research to now focus on shining light on their potential roles in disease. "Once we identify exons that have a critical role in disease, we can use this information to develop new therapies," says Gonatopoulos-Pournatzis. Editing Multiple Genome Fragments At A Time - Source

New System To Edit Multiple Genome Fragments Together

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Editing Multiple Genome Fragments At A Time Multiple sites in the genome can now be edited at the same time, which enables scientists to understand...
How is India fighting Coronavirus

Can India Survive The Coronavirus Attack? Urgent Action Plan Inside

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How is India fighting Coronavirus Coronavirus Outbreak In India / COVID-19 Outbreak in India After China, India - the second most populated country with more...
JNCASR Fellowships Suspension Notice

JNCASR Fellowships Suspension Notice Due to Coronavirus Pandemic

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JNCASR Fellowships Suspension Notice Due to Coronavirus Pandemic Dear BioTecNikans, please read this important notification which has been released by JNCASR with regards to its...
NIBMG Job Openings

Govt NIBMG Project Personnel Recruitment – Rs. 54,000 pm Salary

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NIBMG Job Openings - Life Science/ Genetics/ Biotech Apply NIBMG Job Openings - Life Science/ Genetics/ Biotech Apply. MSc Life Science research jobs at NIBMG....
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