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Biotecnika Times – Newsletter 19.03.2020 – Cancer Center CNCI Job, NCBS...

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Biotecnika Times - Cancer Center CNCI Job, NCBS Graduate Trainee Govt Regional Cancer Center, CNCI Life Science Research Fellow Recruitment Regional Cancer Center Jobs – Life...
Biotech News

Is Coronavirus A Man Made Bioweapon?

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Coronavirus Laboratory Created Or Not - Scientists Provide Evidence According to findings published today in Nature Medicine, the novel coronavirus pandemic that emerged in the Chinese...
Summer Training Program 2020
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Summer Training Program 2020 at Dept of Bioengineering, NIT Agartala

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Summer Training Program 2020 at Dept of Bioengineering, NIT Agartala Summer Training Program 2020 at Dept of Bioengineering, NIT Agartala notification is out. Summer Training...
Editing Multiple Genome Fragments At A Time Scientists can now edit multiple sites in the genome at the same time to learn how different DNA stretches co-operate in health and disease. CRISPR-based DNA editing has revolutionized the study of the human genome by allowing precise deletion of any human gene to glean insights into its function. But one feature remained challenging—the ability to simultaneously remove multiple genes or gene fragments in the same cell. Yet this type of genome surgery is key for scientists to understand how different parts of the genome work together in the contexts of both normal physiology and disease. Now such a tool exists thanks to the teams of Benjamin Blencowe and Jason Moffat, both professors of molecular genetics at the Donnelly Centre for Cellular and Biomolecular Research. Dubbed 'CHyMErA', for Cas Hybrid for Multiplexed Editing and Screening Applications, the method can be applied to any type of mammalian cell to systematically target the DNA at multiple positions at the same time, as described in a study published in the journal Nature Biotechnology. Often described as genome scissors, CRISPR works by sending a DNA-cutting enzyme to desired sites in the genome via guide RNA molecules, engineered to adhere to the target site. The most widely used DNA-cutting enzyme is Cas9. - Editing Multiple Genome Fragments At A Time. Since Cas9 first came to light, other Cas enzymes with distinct properties have been identified by scientists seeking to improve and expand the applications of the technology. Unlike the CRISPR-Cas9 technology, CHyMErA combines two different DNA-cutting enzymes, Cas9 and Cas12a, to allow more versatile applications. Cas12a is an enzyme that can be used to generate multiple guide RNA molecules in the same cell, which is key for simultaneous DNA editing. Thomas Gonatopoulos-Pournatzis, a research associate in Blencowe's group, had spent several years trying to develop combinatorial gene editing by testing Cas9 and Cas12a enzymes on their own. He then had the idea to combine these enzymes to generate the CHyMErA system. "We had been trying a number of approaches to induce genetic fragment deletions and nothing worked as well as CHyMErA," he says. "I was thrilled when together with Shaghayegh Farhangmehr, a Ph.D. student in the Blencowe lab, we saw the first evidence that CHyMErA was successful in deleting gene segments. We obtained these results on Boxing Day and it was the best Christmas present I could have wished for." The next step was to harness CHyMErA in large-scale screens to systematically analyze how genes act together, as well as the functions of individual parts of genes. Blencowe's team, which studies the regulation and function of gene segments known as exons, approached Moffat, whose group had developed extensive experience with CRISPR technology. "With CHyMErA, you can use the best of the two enzymes," says Michael Aregger, a research associate in the Moffat lab, who played a key role in developing the screen-based applications of CHyMErA. "Cas9 has been improved by the community to have a very high editing efficiency, whereas Cas12a allows multiplexing of guide RNAs and therefore provides a lot more flexibility in finding sites in the genome that we can cut." In one application of CHyMErA, the researchers targeted pairs of genes known as paralogs, which have a similar DNA code but remain poorly studied because they were difficult to research. Because paralogs arose by duplication of an ancestral gene, it had been assumed they would largely have similar roles. But their function could not be revealed by the existing single-gene targeting methods typically employed in genetic screens, mostly because the other paralog would compensate for the one that's missing. "With CHyMErA, we can take out both paralogs in pairs to see if that ancestral function is important for the cell to survive," says Kevin Brown, a senior research associate in the Moffat lab and co-lead author on the study along with Aregger and Gonatopoulos-Pournatzis. "We are able to now interrogate a class of genes that were previously missed." After knocking out ~700 paralog pairs, almost all that exist in the human genome, the analysis confirmed that many of these gene pairs do indeed perform similar roles in cell survival, whereas others have distinct functions. Another feature of CHyMErA is that both Cas9 and Cas12a can be deployed to nearby genome sites to cut out gene fragments such as exons. This allowed the team to individually delete thousands of exons that have been linked to cancer and brain function but were not amenable to targeting with Cas9 alone. Exons are variably included in genes' transcripts and can modify the function of the encoded proteins, although how individual exons contribute to cellular processes remains largely unknown. Out of 2,000 exons analyzed by CHyMErA, over 100 were found to be critical for cell survival, enabling future research to now focus on shining light on their potential roles in disease. "Once we identify exons that have a critical role in disease, we can use this information to develop new therapies," says Gonatopoulos-Pournatzis. Editing Multiple Genome Fragments At A Time - Source
Biotech News

New System To Edit Multiple Genome Fragments Together

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Editing Multiple Genome Fragments At A Time Multiple sites in the genome can now be edited at the same time, which enables scientists to understand...
How is India fighting Coronavirus
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Can India Survive The Coronavirus Attack? Urgent Action Plan Inside

Prathibha HC - 0
How is India fighting Coronavirus Coronavirus Outbreak In India / COVID-19 Outbreak in India After China, India - the second most populated country with more...
JNCASR Fellowships Suspension Notice
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JNCASR Fellowships Suspension Notice Due to Coronavirus Pandemic

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JNCASR Fellowships Suspension Notice Due to Coronavirus Pandemic Dear BioTecNikans, please read this important notification which has been released by JNCASR with regards to its...
NIBMG Job Openings
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Govt NIBMG Project Personnel Recruitment – Rs. 54,000 pm Salary

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NIBMG Job Openings - Life Science/ Genetics/ Biotech Apply NIBMG Job Openings - Life Science/ Genetics/ Biotech Apply. MSc Life Science research jobs at NIBMG....
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Biotecnika Times – Newsletter 18.03.2020 – Govt Armed Forces MSc Job,...

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Biotecnika Times - Govt Armed Forces MSc Job, Govt Food Officer Job Govt Armed Forces Medical College MSc Recruitment 2020 Government Armed Forces Medical College Senior...
IIT Delhi PhD Admission
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IIT Delhi PhD Admission 2020 – Applications Invited

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IIT Delhi PhD Admission 2020 - Applications Invited The official notification for the IIT Delhi PhD Admission 2020 - Applications Invited has been released. Interested...
Coronavirus COVID-19 vaccine
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U.S Volunteer Gets First Shot Of Coronavirus COVID-19 Vaccine

Namitha Thampi - 0
A volunteer from the U.S gets the first shot of Coronavirus COVID-19 vaccine Researchers from UNITED STATE gave the very first shot of the experimental...
New Approach to Speed up RBCs generation
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Scientists At NCCS Pune Develop New Approach to Speed up RBCs...

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New Approach to Speed up RBCs generation in the Laboratory For a number of conditions like blood-related cancers,  solid malignancies, pregnancy-related complications, transplant surgery, supportive...
NCCS PhD Admission 2020
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NCCS PhD Admission 2020 August – Applications Invited

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NCCS PhD Admission 2020 August - Applications Invited NCCS, Pune has announced the PhD Admission - August 2020. Only post graduate students with a valid...
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Six Months Project Training at National Institute of Immunology – Applications...

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NII Project Training 2020 - Six Months, Applications Invited Six Months Project Training at National Institute of Immunology - Applications Invited. Project Training Between July...
BioTecNika Times

Biotecnika Times – Newsletter 17.03.2020 – CSIR NET June 2020 Notification,...

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Biotecnika Times - CSIR NET June 2020 Notification, UPSC Govt Job CSIR NET June 2020 Official NTA Notification – Online Applications Open The NTA will conduct...
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Confirmed Coronavirus Cases In India Rise To 114 – Maharashtra’s...

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Confirmed coronavirus cases rise to 114 in India as Maharashtra's numbers rise The rise in coronavirus COVID-19 positive cases in Maharashtra prompted CM Uddhav Thackeray's...
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CSIR NET June 2020 Official NTA Notification – Online Applications Open

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CSIR NET June 2020 Official NTA Notification - Online Applications Open JOINT CSIR-UGC NET JUNE-2020 PUBLIC NOTICE (16 March 2020) This call expires in : This Call Has Expired   The...
Asymptomatic people spread Coronavirus
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Coronavirus: Can Asymptomatic People Spread COVID-19?

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Asymptomatic People Spread Coronavirus - Covid-19 Biggest Concerns COVID-19 Global Outbreak Increase Health officials are urging people and communities to take precautions and do their maximum...
Coronavirus Testing Sites India
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52 Coronavirus COVID-19 Testing Sites In India

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Coronavirus COVID ‐19 Testing Sites In India Coronavirus in India In India, the Coronavirus COVID-19 pandemic has initially spread to India on 30 January 2020 from...
ICMR-NIMR PhD Programme Admission
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ICMR-NIMR PhD Programme Admission 2020-2021 : Applications Invited

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ICMR-NIMR PhD Programme Admission 2020-2021 : Applications Invited ICMR-NIMR PhD Programme Admission 2020-2021 : Applications Invited. ICMR-National Institute for Malaria Research Admission to Ph.D. Programme;...
NCCS Project Training 2020
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NCCS Project Training 2020 Biological Science – Applications Invited

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NCCS Project Training 2020 Biological Science - Applications Invited MSc and MTech Project Training Programme 2020 at NCCS, Pune. NCCS Pune Project Training for MSc...
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