Buy Chemiluminescent HRP Substrates Online For Lab Research

Buy Chemiluminescent HRP Substrates Online For Lab Research

Horseradish peroxidase (HRP), ß-Galactosidase (BGAL) are Enzyme Substrates for reporter enzymes commonly used For research purpose. Substrates are available for each enzyme which can be either a soluble substrate or insoluble substrate. Wherein soluble substrate help to quantitate analytes in a liquid phase assay like ELISA and to localize analytes in a solid phase insoluble substrate is used, eg in western blotting. Chemiluminescent substrates are available for horseradish peroxidase for ELISA and Western Blots.


Chemiluminescent HRP substrates

Most of the Chemiluminescent HRP substrates manufactured by biosuppliers are highly sensitive thus producing accurate results and are nonradioactive too. Luminol-based chemiluminescent substrates are manufactured for use in both western blotting (WB) and enzyme immunoassay (ELISA).


Rockland Antibodies & Assays

Rockland manufactures two types of chemiluminescent HRP substrates with different sensitivity level.

  • FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate  – For detecting femtogram (10-15) levels of antigen.
  • PicoMax™ Sensitive Chemiluminescent HRP Substrate – For detecting picoogram (10-12) l levels of antigen.


Merck provides a product called as Immobilon Western Chemiluminescent HRP Substrate. With its high level of sensitivity for Chemiluminescent detection in western or blotting applications on both PVDF and nitrocellulose membranes, it is very effective & result oriented. Its compatibility with all commonly used buffers and blocking reagents is commendable.

Immobilon Western Chemiluminescent HRP Substrate


Product NameChemiluminescent HRP Substrate (membrane)

Features: The Abcam Chemiluminescent HRP Substrate (membrane) is quite sensitive and while performing research in order to maximize individual assay components and minimize background reactivity extreme care needs to be taken.


Searchlight Ultra-Sensitive Chemiluminescent HRP Membrane/Blotting ECL Substrate is being manufactured by RayBiotech. Features are mentioned below:

  • To be used for horseradish peroxidase (HRP) based membrane blotting applications.
  • This substrate is specially designed for HRP membrane/dot blotting and Western blotting applications.
  • High sensitivity: low Pico to Mid Femto detection
  • High signal to noise ratio
  • 24-month shelf life
  • A mixed substrate is stable for several days


Thermo Fisher

Thermo Fisher is the leading Manufactures of its own set of chemiluminescent substrates. Recommended chemiluminescent substrates are mentioned below along with its usage.


Pierce ECL substrate Pierce ECL Plus substrate SuperSignal West Pico PLUS substrate SuperSignal West Dura substrate SuperSignal West Femto substrate
Detection level Low- to midpicogram Low-picogram Low-picogram to high-femtogram Mid-femtogram Low- to midfemtogram
Signal duration 0.5–2 hr 5 hr 6–24 hr 24 hr 8 hr
Detection methods X-ray film, CCD imager X-ray film, CCD imager, fluorescence imager X-ray film, CCD imager X-ray film, CCD imager X-ray film, CCD imager
Select when … .. target and sample are abundant … target is less abundant, sample is limited, and you need chemifluorescent detection … target is less abundant, sample is limited, and you need more sensitivity than an entry-level ECL substrate provides … target is less abundant, sample is limited, and you are using CCD image capture … target is least abundant, sample is precious, and you need maximum sensitivity
Value to you Low cost; easy to switch from other entry-level ECL substrates Best detection flexibility with chemifluorescent detection option Best value;
works for majority of western blots
Best signal duration Best sensitivity



Keywords: Buy Chemiluminescent HRP Substrates, Price of Buy Chemiluminescent HRP Substrates, Buy Buy Chemiluminescent HRP Substrates Online, Chemiluminescent HRP Substrates For Lab Research Use, Buy Chemiluminescent HRP Substrates at a low price, Order Chemiluminescent HRP Substrates Online. Buy Chemiluminescent HRP Substrates from biosuppliers at cheaper rates.

Perfection is her hobby, Reliability is a synonym, Editing is her passion, Excellence is her Goal, Tactfulness is in her genes, Yellow is her Fav color. Preety is the name of the Professional on whom entire BioTecNika relies when it comes to its website. A Gold Medalist in Biotech from SRM University, Chennai with a 9.9 CGPA ( was awarded the Gold Medal by Honorable Prime Minister of India Shri Narendra Modi , as seen in the pic ), She decided to join forces with BioTecNika to ensure India's largest BioSciences Portal expands its reach to every city in India. She has redesigned the new avatar of BioTecNika from scratch and heads the most dynamic, vibrant and well informed Online Team at Biotecnika Info Labs Pvt Ltd