Buy Chemiluminescent HRP Substrates Online For Lab Research
Horseradish peroxidase (HRP), ß-Galactosidase (BGAL) are Enzyme Substrates for reporter enzymes commonly used For research purpose. Substrates are available for each enzyme which can be either a soluble substrate or insoluble substrate. Wherein soluble substrate help to quantitate analytes in a liquid phase assay like ELISA and to localize analytes in a solid phase insoluble substrate is used, eg in western blotting. Chemiluminescent substrates are available for horseradish peroxidase for ELISA and Western Blots.
Chemiluminescent HRP substrates
Most of the Chemiluminescent HRP substrates manufactured by biosuppliers are highly sensitive thus producing accurate results and are nonradioactive too. Luminol-based chemiluminescent substrates are manufactured for use in both western blotting (WB) and enzyme immunoassay (ELISA).
Rockland Antibodies & Assays
Rockland manufactures two types of chemiluminescent HRP substrates with different sensitivity level.
- FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate – For detecting femtogram (10-15) levels of antigen.
- PicoMax™ Sensitive Chemiluminescent HRP Substrate – For detecting picoogram (10-12) l levels of antigen.
Merck provides a product called as Immobilon Western Chemiluminescent HRP Substrate. With its high level of sensitivity for Chemiluminescent detection in western or blotting applications on both PVDF and nitrocellulose membranes, it is very effective & result oriented. Its compatibility with all commonly used buffers and blocking reagents is commendable.
Product Name: Chemiluminescent HRP Substrate (membrane)
Features: The Abcam Chemiluminescent HRP Substrate (membrane) is quite sensitive and while performing research in order to maximize individual assay components and minimize background reactivity extreme care needs to be taken.
Searchlight Ultra-Sensitive Chemiluminescent HRP Membrane/Blotting ECL Substrate is being manufactured by RayBiotech. Features are mentioned below:
- To be used for horseradish peroxidase (HRP) based membrane blotting applications.
- This substrate is specially designed for HRP membrane/dot blotting and Western blotting applications.
- High sensitivity: low Pico to Mid Femto detection
- High signal to noise ratio
- 24-month shelf life
- A mixed substrate is stable for several days
Thermo Fisher is the leading Manufactures of its own set of chemiluminescent substrates. Recommended chemiluminescent substrates are mentioned below along with its usage.
|Pierce ECL substrate||Pierce ECL Plus substrate||SuperSignal West Pico PLUS substrate||SuperSignal West Dura substrate||SuperSignal West Femto substrate|
|Detection level||Low- to midpicogram||Low-picogram||Low-picogram to high-femtogram||Mid-femtogram||Low- to midfemtogram|
|Signal duration||0.5–2 hr||5 hr||6–24 hr||24 hr||8 hr|
|Detection methods||X-ray film, CCD imager||X-ray film, CCD imager, fluorescence imager||X-ray film, CCD imager||X-ray film, CCD imager||X-ray film, CCD imager|
|Select when …||.. target and sample are abundant||… target is less abundant, sample is limited, and you need chemifluorescent detection||… target is less abundant, sample is limited, and you need more sensitivity than an entry-level ECL substrate provides||… target is less abundant, sample is limited, and you are using CCD image capture||… target is least abundant, sample is precious, and you need maximum sensitivity|
|Value to you||Low cost; easy to switch from other entry-level ECL substrates||Best detection flexibility with chemifluorescent detection option||Best value;
works for majority of western blots
|Best signal duration||Best sensitivity|
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