Novel Gene Therapy Approach with MOF-Mediated CRISPR/Cas9 Delivery
Scientists from RMIT University applied a new strategy with combined effects of metal-organic frameworks (MOFs) and phytochemical layering from green tea for targeting human prostate cancer cells, a novel gene therapy approach
The new technique for deploying the genetic engineering instrument right into cells of interest is a significant move in the direction of a lot more reliable, secure, and also less expensive genetic remedies with therapy options for numerous genetic disorders.
Owing to the versatility and presence of biologically compatible nanomaterials, Ravi Shukla, Lead investigator, and associate professor stated that this novel gene therapy approach was a cogent substitute for the prevalent viral mechanisms for presenting the gene snipping tool CRISPR/Cas9.
Compared to the viral method, non-viral MOFs provide a secure option for patients and have the potential to transport massive gene contents, he added.
Nobel Laureate subject in Chemistry, 2020, CRISPR/Cas9, extensively addressed as a quantum leap in genetic engineering for its talent in elimination and replacement of faulty DNA, nevertheless, the most significant challenge continues to be properly supplying it to cells.
There are presently simply 13 accepted approaches in trial worldwide and all count on viral therapies, a strategy which is both expensive and has health threats.
RMIT had collaborated with the CSIRO who have earlier established a modern technology to transport and shield biomolecules with MOFs.
CSIRO Study Team Leader, Dr. Cara Doherty, mentioned that the CSIRO had also presented techniques to produce industrial level quantities of different MOFs, with the possibility to considerably decrease the price of launching it to market.
They’re excited to discover a novel method through which these compounds can tackle intricate biological concerns, including targeting certain therapeutic functions like gene therapy, she claimed.
Cells are not made to naturally take up new genes or DNA products, and also the threats interlinked with presenting a virus right into the body have reduced the progress of study to viral approaches.
To additionally ameliorate the MOF’s potential to get in the host cell, it was layered with phytochemical material present in green tea, named epigallocatechin-gallate (EGCG), familiar for its anticancer and antioxidant features
Co-author Arpita Poddar told that the ECG operated by binding to the upper area of the MOF, helping it to penetrate inside the host cells.
We observed a rise in the cell intake of over 23% for ECG layered MOFs with respect to regular ones, she said.
The recent findings were based on the study performed earlier by the same group who created evidence for the delivery model.
The coming objective is to experiment with the pertained mechanism for its uses in acting against numerous pathogenic genes.
The journal mentioning the novel gene therapy approach, ZIF-C for Targeted RNA Interference and CRISPR/Cas9 Based Gene Editing in Prostate Cancer, is issued in ChemComm.
Author : Geema George