Freshers Job for MSc Biotechnology, Biochemistry, Microbiology at Lupin
Lupin Pharmaceuticals is hiring MSc Freshers. Freshers Job for MSc Biotechnology, Biochemistry, Microbiology at Lupin. Interested Candidates can check out the details below and Apply Online.
Job Post: Officer -Production (Biotech)
Job Location: Pune Biotech, MH, IN
Job Company: Lupin
Job Description :
1. Preparation of GMP documents like SOPs, qualification and validation protocols, reports and other process or facility related documents for media preparation and cell culture operations.
2. Operation of disposable and stainless steel bioreactors, sub culture and cell banking operations.
3. Execution of media, feed preparation and steam sterilizer operations.
4. In- process recording of process parameters and In-process sample analysis.
5. CIP and SIP of bioreactors.
6. Continuous updation and archival of area/equipment related documents.
7. Checking of filter integrity, calibration and maintenance of pH meter, conductivity meter and osmometer.
Education – M.Sc.(Biotechnology. Biochemistry, Microbiology)
Experience – 0 to 2 years
Here are five interview questions along with sample answers for the position of Officer – Production (Biotech) at Lupin:
1. Question: Can you describe your experience with GMP document preparation and its importance in biotech production?
Answer: GMP document preparation is critical in ensuringthe quality and compliance of biotech production processes. In my previous role as a research assistant at XYZ Biotech, I was responsible for creating SOPs, validation protocols, and reports for various processes, including media preparation and cell culture operations. These documents are essential as they provide a standardized framework for operations, ensuring consistency and adherence to regulatory standards. I understand the significance of maintaining accurate and up-to-date GMP documents to facilitate smooth production operations and regulatory compliance.
2. Question: How familiar are you with the operation of disposable and stainless steel bioreactors? Can you provide an example of a challenging situation you’ve encountered while operating them and how you resolved it?
Answer: I have experience operating both disposable and stainless steel bioreactors during my academic studies and internships. One challenging situation I encountered was a sudden pressure drop in a stainless steel bioreactor during a fermentation process. I quickly identified a leak in one of the gaskets, which was causing the pressure drop. I followed the standard operating procedure for equipment troubleshooting, isolated the bioreactor, replaced the gasket, and resumed the operation. This incident emphasized the importance of regular equipment maintenance and troubleshooting skills in ensuring the continuity of production processes.
3. Question: Can you walk us through the steps involved in steam sterilizer operations, and what precautions should be taken to ensure sterilization efficacy?
Answer: Steam sterilization is a critical step in biotech production to eliminate contaminants. The key steps include loading the equipment correctly, setting the appropriate temperature and pressure parameters, and ensuring proper steam penetration throughout the load. To ensure sterilization efficacy, it’s crucial to perform routine equipment maintenance, such as calibration of temperature and pressure gauges, and verify that all load items are compatible with steam sterilization. Additionally, regular validation of sterilization cycles and documentation of each cycle’s parameters are essential to demonstrate compliance with GMP standards.
4. Question: How do you perform in-process recording of process parameters, and why is it important in biotech production?
Answer: In-process recording involves monitoring and documenting various parameters during biotech production to ensure that the processes are running within specified ranges. This includes parameters like temperature, pH, agitation speed, and oxygen levels. I use calibrated instruments to collect real-time data and record it accurately in batch records or electronic systems. In-process recording is vital because it allows for immediate detection of deviations from the desired process conditions, enabling timely corrective actions. It also serves as a historical record for traceability and process improvement.
5. Question: Can you explain the significance of CIP (Clean-in-Place) and SIP (Sterilize-in-Place) procedures in bioreactor operations, and how would you ensure their proper execution?
Answer: CIP and SIP procedures are crucial for maintaining the cleanliness and sterility of bioreactors. CIP ensures that all product residues and contaminants are effectively removed after a production run, while SIP sterilizes the bioreactor to prepare it for the next batch. To ensure proper execution, I would strictly follow validated CIP and SIP protocols, ensuring that all cleaning agents and sterilization parameters are correctly applied. I would also conduct regular inspections of equipment and review historical data to confirm the effectiveness of these procedures, thus guaranteeing the integrity of the bioreactors and the safety of the biotech production process.
These sample questions and answers should help you prepare for your interview for the Officer – Production (Biotech) position at Lupin. Be sure to tailor your responses to your specific experiences and qualifications.
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