ACTREC Life Sciences Research Fellow Job Opening – Attend Walk-In-Interview

Here are 5 possible interview questions along with their answers for the given job description:

Question 1: Can you explain your experience in experimental biology and how it relates to the techniques mentioned in the job description? Answer: Certainly. I have hands-on experience in various experimental techniques, including mammalian tissue culture handling, gene cloning and expression, transient transfections, lentivirus production, stable gene expression and knock-down, cell viability assays, Western-blot analysis, QPCR, and immunofluorescence staining. My practical familiarity with these techniques has been gained through both academic coursework and hands-on laboratory work.

Question 2: Could you provide an example of a project where you effectively utilized cell viability assays and Western-blot analysis? Answer: Of course. In a previous project, I was investigating the impact of a novel drug candidate on cancer cell viability. I conducted cell viability assays to assess the drug’s effect on cell survival. Additionally, I employed Western-blot analysis to evaluate changes in key protein expression levels in response to the treatment, helping to uncover potential mechanisms underlying the drug’s activity.

Question 3: How comfortable are you with using lentivirus production for stable gene expression and knock-down? Can you elaborate on your experience in this area? Answer: I am quite comfortable with lentivirus production and its applications. In my master’s research, I designed and executed experiments involving lentivirus-mediated stable gene expression and knock-down in specific cell lines. This approach allowed me to investigate gene functions and their impact on cellular pathways, contributing to a deeper understanding of cellular processes.

Question 4: Have you worked with animal models such as mice? Could you discuss your experience with mice modelling and tissue-based analysis? Answer: Yes, I have experience with mice modelling. During my research project, I developed and managed a mouse model to study the effects of a certain treatment on tumour growth. I conducted tissue-based analysis, extracting and analyzing relevant tissues for changes in gene expression and other molecular markers. This experience provided valuable insights into translating in vitro findings to in vivo scenarios.

Question 5: Can you share details about any research articles you’ve published related to the techniques mentioned in the job description? Answer: Certainly. I have authored a research article that focuses on the role of a specific gene in breast cancer progression. In this study, I applied techniques such as gene cloning, QPCR, and immunofluorescence staining to investigate the gene’s expression patterns in cancer cells. The article contributes to the understanding of breast cancer biology and highlights my proficiency in these techniques.

Remember, these answers are designed to provide a general idea of how to respond to the interview questions. Candidates should tailor their answers to their own experiences and accomplishments.