CSIR NET Life Science Enzymes Notes
Today’s Do Not Skip CSIR NET Topic deals with a crucial topic of the CSIR NET Life science exam: Enzymology -Enzyme Inhibition. Revise faster with these expert-designed mind maps for Enzyme Inhibition. Also, understand:
- The importance of this topic – Enzyme Inhibition as per the CSIR NET Exam
- What are the important concepts you must learn & revise from Enzyme Inhibition Topic
- How many marks questions usually come in the CSIR NET exam on this topic?
- Types of questions asked
- Reference Books
- Download CSIR NET Enzyme Inhibition Fast Revision Notes / Mind Map
Inhibition, in enzymology, is a phenomenon in which a compound, called an inhibitor, in most cases similar in structure to the substance (substrate) upon which an enzyme acts to form a product, interacts with the enzyme so that the resulting complex either cannot undergo the usual reaction or cannot form the usual product.
Enzyme inhibitors prevent the formation of an enzyme-substrate complex and hence prevent the formation of the product.
- Inhibition of enzymes may be either reversible or irreversible depending on the specific effect of the inhibitor is used.
- The inhibitor may function by combining with the enzyme at the site where the substrate usually combines (competitive inhibition) or at some other site (noncompetitive inhibition).
- In the latter, the inhibitor does not prevent the binding of the substrate to the enzyme. Still, it sufficiently changes the site’s shape at which catalytic activity occurs to prevent it.
CSIR NET Life Science Enzymes Notes
Enzyme inhibitors can serve a variety of purposes, including in medicine (to treat disease) and agriculture (as pesticides)
- An example of a use for a competitive inhibitor is in the treatment of influenza via the neuraminidase inhibitor, Relenza
- An example of a use for a non-competitive inhibitor is the use of cyanide as a poison (prevents aerobic respiration)
Important concepts to learn & revise from Enzyme Inhibition :
1. Concept of normal enzyme mechanism
2. Differentiating with the inhibited enzyme mechanism
3. Types of inhibitors with examples from metabolism subunit
4. Understanding enzyme kinetics of regular and inhibited enzymes
- Knowledge about MM equation, its modifications
- Concept of Km app and Vmax app and related terms
- Calculating different enzyme kinetics parameters using the formulas
- Knowing the graphs of each type of inhibition
Types of questions asked related to Enzyme Inhibition in CSIR NET Exam :
- Graph-based questions to identify the type of inhibition from a given LB plot/MM plot
- True/False statements regarding the mechanism of action of different inhibition types
- Match the following questions to connect different inhibitors with the enzymes
- Related questions with biomolecule metabolism (especially carbohydrate metabolism, ETC ) about different enzymes and their activators/inhibitors
- Numerical type questions to calculate the different parameters such as Vmax or km for enzyme inhibited reactions
Weightage of this topic – Enzyme Inhibition in the CSIR NET exam :
- On average, questions from the entire enzymology are asked in every exam for at least 12-14 marks, including parts B and C.
- Specifically, 1-2 enzyme inhibition questions can be expected
Reference books for this topic :
- Lehninger Principles of Biochemistry by David L Nelson
- Biochemistry: International Edition by Stryer, Berg and Tymoczko
- Principles of Biochemistry by Voet, Pratt, and Voet
- ENZYMES: Biochemistry, Biotechnology, Clinical Chemistry, 2/E by Trevor Palmer and Philip Bonner.