The DNA vital to the life of a cell is wrapped in chromosomes, and a variety of barriers, repair mechanisms, and other cellular defences exist to maintain the integrity of the chromosomes during cell growth and division. These safeguards can however fail, and a cell may find itself trying to divide into two daughter cells with a loose chromosomal fragment wandering away from a broken chromosome.
William Sullivan calls this a “worst case scenario” for the cell. The potential consequences include cell death or an uncontrolled cell growth. But Sullivan, a professor of molecular, cell, and developmental biology at UC Santa Cruz, is of the opinion that the cell still has one more lifeboat rescue the broken chromosome.
The latest results from Sullivan’s lab, published in the June 5 issue of Journal of Cell Biology, disclose new aspects of an outstanding mechanism that carries broken chromosomes through the process of cell division so that they can be repaired and function normally in the daughter cells. Sullivan has been studying this process in the fruit fly Drosophila melanogaster. His lab has created a strain of flies in which broken chromosomes are common due to the expression of a DNA-cutting enzyme.
We have
flies in which 80 per cent of the cells have double-strand breaks in the DNA, and the flies are fine,” he said. “The cell has this amazing mechanism, like a Hail Mary pass with time running out.”The mechanism involves the creation of a DNA rope which acts as a bridge to keep the broken fragment connected to the chromosome. Powerful new microscopy techniques enable researchers to observe the whole process in living cells, with bright fluorescent tags highlighting the chromosomes and other cellular components.
During cell division, cell duplicates its chromosomes to make one copy for each of the daughter cells. The membrane around the nucleus, which keeps the chromosomes separate from the rest of the cell, breaks down. The two sets of chromosomes then line up and segregate to opposite sides of the cell, pulled apart by a structure of microtubules called the spindle fibres. A new nuclear envelope forms around each set of chromosomes, and new cell membranes separate the two daughter cells.
Sullivan’s research has shown that chromosome fragments don’t segregate with the rest of the chromosomes, but get pulled in later just before the newly forming nuclear membrane closes. “The DNA tether seems to keep the nuclear envelope from closing, and then the chromosome fragment just glides right in at the last moment,” Sullivan said.
If this mechanism fails, however, and the chromosome fragment gets left outside the nucleus, the consequences are dreadful. The fragment forms a “micronucleus” with its own membrane and becomes susceptible to extensive relocations of its genetic material, which can then be reincorporated into chromosomes during the next cell division. Micronuclei and genetic rearrangements are commonly seen in cancer cells.
“We want to understand the mechanism that keeps that from happening,” Sullivan said. “We are currently identifying the genes responsible for generating the DNA tether, which could be promising novel targets for the next generation of cancer therapies.”
