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Life Sciences Jobs at Sanzyme Biologics
Explore exciting Life Sciences Jobs at Sanzyme Biologics, a global leader in probiotics and fermentation-derived nutritional ingredients. Sanzyme Biologics is hiring talented professionals to join their team in Hyderabad, offering opportunities to work on cutting-edge biotechnology, downstream processing, and innovative bioproduct development. Whether you are an experienced professional or a fresher with a passion for biotech, these roles provide hands-on experience in state-of-the-art facilities. Apply now to advance your career in life sciences with Sanzyme Biologics.
Job Role: Biomolecules Downstream Processing Specialist
Company: Sanzyme Biologics
Location: Hyderabad, Telangana
Experience: 3+ years
Salary: Depends on last CTC
Company Overview
Sanzyme Biologics, originally founded as Uni-Sankyo in 1969, is a global leader in probiotics and fermentation-derived nutritional ingredients. With over five decades of expertise, the company develops high-quality, shelf-stable probiotic strains and enzymes for food, pharmaceutical, nutraceutical, and agricultural applications. Headquartered in Hyderabad, India, Sanzyme Biologics operates state-of-the-art, ISO-, GMP-, Halal-, and Kosher-certified facilities, and supplies science-backed products to customers in more than 40 countries. The company is dedicated to improving human and animal health through innovation, quality, and advanced biotechnology.
Role Description
Sanzyme Biologics is seeking a Biomolecules Downstream Processing Specialist with strong expertise in the purification of recombinant proteins, enzymes, peptides, and biologics (primarily expressed in E. coli). This is a full-time, on-site role based in Hyderabad. The ideal candidate will have hands-on experience in designing, optimizing, and scaling downstream purification processes—from bench scale to commercial manufacturing—under cGMP environments.
Key Responsibilities
Primary Recovery
• Optimize cell harvest operations (centrifugation, microfiltration)
• Execute high-efficiency cell lysis (homogenization, sonication, chemical/enzymatic methods)
• Handle inclusion bodies: isolation, washing, solubilization
Protein Refolding & Solubilization
• Solubilize proteins using chaotropic agents (urea, guanidine HCl)
• Perform high-throughput screening for refolding conditions
• Execute redox-controlled refolding for disulfide-bond-containing proteins
Chromatography Expertise
• Operate and optimize: Affinity (His-tag, GST, Protein A/G, IMAC), Ion exchange (IEX), Hydrophobic interaction (HIC), Size-exclusion (SEC), Mixed-mode chromatography
• Develop multistep purification workflows
Filtration Technologies
• Perform MF and UF/DF operations
• Conduct buffer exchange and concentration
• Scale filtration systems (flat sheet, hollow fiber, TFF)
Process Development & Scale-Up
• Conduct Design of Experiments (DoE)
• Perform process robustness and characterization studies
• Implement viral inactivation (where applicable) and endotoxin control
• Support tech transfer to pilot and commercial manufacturing sites
Quality & Regulatory
• Work within cGMP operations
• Prepare and maintain batch records, SOPs, validation documents
• Conduct purity, potency, impurity profiling
• Contribute to endotoxin reduction strategies and QC method development
Qualifications
• MSc / MTech / BTech in Biotechnology, Biochemistry, or related Life Sciences (Master’s preferred)
• Strong expertise in downstream processing: chromatography, filtration, centrifugation, and precipitation
• Hands-on experience with analytical and QC methods
• Demonstrated ability in process optimization and troubleshooting
• Knowledge of regulatory and compliance standards in manufacturing environments
• Excellent analytical thinking, problem-solving abilities, and attention to detail
• Strong collaboration skills and ability to work in multidisciplinary teams
• Prior experience in probiotics, fermentation, or related biotech fields is a plus
