NIAB Job 2023 Biotech, Biochem, Life Sciences & Microbiology
NIAB Job 2023 Biotech, Biochem, Life Sciences & Microbiology. National Institute of Animal Biotechnology vacancies. MSc, MTech Biochem, Microbiology, Life Sciences and Biotechnology project jobs. Interested and eligible applicants can check out all of the details on the same below
Don’t forget to check out possible interview questions for this job below
This job expires in
National Institute of Animal Biotechnology, Hyderabad–500032
(An Autonomous Institute of the Department of Biotechnology)
Advt. No. 19/2023
Applications are invited from suitable candidates for filling up the following positions at National Institute of Animal Biotechnology (NIAB), Hyderabad.
Name of the Post: Project Associate-I
No. of Posts: 1 Position
Name of the Project: Therapeutic protein production in milk of farm animals to increase their affordability.
Tenable Upto: September 2024.
Name of the PI: Dr.Nirmalya Ganguli.
Age: 35 years (Relaxation is admissible in case of SC/ST/OBC candidates as per Government instructions).
How to Apply –
Applications are to be filled ONLINE. Online link for submission of application is available from 08-06-2023 and the last date is 23-06-2023 by 5 PM. Candidates must fill online application form at https://www.niab.res.in in sufficient time before the last
date to avoid any last minute technical issues. Suitable candidates will be screened, intimated through email and interviewed by duly constituted selection committee through online mode. Original documents in support of date of birth, educational qualifications and experience certificates are required to be submitted at time of joining. No need to send hard copy. Interim enquiries will not be entertained.Fellowship amount: Rs. 25000/-PM+ HRA as per the DST guidelines.
Eligibility: Post Graduate Degree in Life Sciences (Biotechnology, Biochemistry, Microbiology, Etc.) or Post Graduate degree in professional courses (M. Tech in Life Sciences, M. VSc. Etc.).
Experience: Candidate with prior experience in any of the following area will be preferred.
- Various molecular biology techniques, like RT-PCR, QRT-PCR, Immuno
histochemistry, Immune cytochemistry, Droplet Digital PCR, protein purification and
western blot analysis, - Generation of expression vector by molecular cloning and its invitro characterisation,
culture andmaintenance of cell lines, - Generation of expression vector for genome editing (knock-out and knock-in) using
CRISP – Cas9tools, - Regulation of gene expression by shRNA mediated knock down,
- Cell sorting by FACS/MACS, single cell sorting and generation of cell lines,
- Isolation and culture of Stem cells, transfection and maintenance of stem cells,
generation of iPSC,isolation and culture of cells from different tissue sources, - Signal Transduction study by functional genomics.
- Handling and experimentation on rodent and goat model
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💡 Here are a few possible interview questions for the above job posting and their answers.
👍We hope this helps in giving you a rough idea as to how to prepare for the interview for this specific role. Good luck!
- Question: Can you describe your experience with molecular biology techniques such as RT-PCR, QRT-PCR, and protein purification? Answer: During my previous research projects and academic studies, I have extensively used molecular biology techniques including RT-PCR, QRT-PCR, and protein purification. I am familiar with experimental design, primer design, optimization of PCR conditions, data analysis, and protein purification techniques such as column chromatography.
- Question: Have you worked with CRISPR-Cas9 genome editing? If yes, could you explain your experience in generating expression vectors for genome editing and the subsequent characterization? Answer: Yes, I have hands-on experience with CRISPR-Cas9 genome editing. In my previous project, I successfully generated expression vectors for genome editing using CRISPR-Cas9 tools. I performed molecular cloning to create the vectors and conducted in vitro characterization to confirm their functionality. I also analyzed the edited genomic regions to assess the efficiency of knock-out and knock-in events.
- Question: Can you discuss your experience with cell sorting techniques such as FACS/MACS and single-cell sorting? Additionally, have you generated stable cell lines? Answer: I have practical experience in cell sorting techniques, including FACS/MACS and single-cell sorting. I have used these techniques to isolate specific cell populations for downstream analysis or experimentation. Furthermore, I have generated stable cell lines by transfecting cells with expression vectors carrying selectable markers and conducting appropriate selection procedures.
- Question: Could you elaborate on your experience with stem cell isolation and culture, as well as the generation of induced pluripotent stem cells (iPSCs)? Answer: I have hands-on experience in isolating and culturing stem cells from various tissue sources. I have worked with both adult stem cells and iPSCs. I am familiar with techniques for maintaining the pluripotency and self-renewal of iPSCs, as well as inducing their differentiation into specific cell lineages. I have also conducted experiments involving the characterization and functional analysis of stem cells.
- Question: Have you conducted experiments using animal models? If yes, can you discuss your experience with rodent and goat models and the specific techniques you have employed? Answer: Yes, I have experience in handling and conducting experiments using rodent and goat models. I have performed various techniques such as sample collection, administering treatments or interventions, and monitoring physiological and biochemical parameters. I am well-versed in maintaining animal welfare and following ethical guidelines during experimentation.
Please note that these are sample answers, and it’s important to tailor your responses to your own experiences and knowledge. Good luck with your interview!
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