CSIR-IMTECH Molecular Biology Project Associate Job, Apply Online

1. Can you describe your experience in research and development in academic institutions or science and technology organizations? How have you contributed to scientific activities and services?
   • Answer: In my previous role at an academic institution, I actively participated in research projects related to cancer biology. I conducted experiments, analyzed data, and contributed to scientific publications. Additionally, I collaborated with fellow researchers and provided scientific support to ongoing projects. I also engaged in scientific outreach activities, such as organizing seminars and presenting research findings at conferences, to contribute to the scientific community.
2. How familiar are you with cell culture techniques? Can you provide examples of how you have utilized cell culture in your previous work?
   • Answer: I have hands-on experience with cell culture techniques, including cell line maintenance, passage, and experimentation. In my previous research project, I worked with breast and gynecological cancer cell lines, performing assays to evaluate the effects of different compounds on cell viability and proliferation. I followed strict aseptic techniques, optimized culture conditions, and ensured accurate documentation of experimental procedures and results.
3. Have you worked with recombinant protein expression and characterization? Can you elaborate on your experience in this area?
   • Answer: Yes, I have experience in recombinant protein expression and characterization. In a previous project, I designed and constructed expression vectors for specific proteins of interest. I performed transfection experiments in mammalian cell lines and optimized protein expression conditions. Subsequently, I purified the recombinant proteins using chromatographic techniques and conducted characterization assays, such as SDS-PAGE and Western blotting, to assess their integrity and functionality.
4. How proficient are you in molecular biology techniques, particularly gene expression analysis? Please provide examples of your expertise in this field.
   • Answer: Molecular biology techniques are integral to my skill set. I have conducted gene expression analysis using quantitative PCR (qPCR) and RT-PCR methods. In one of my projects, I investigated the expression patterns of key genes associated with breast cancer progression. I extracted RNA from cell lines and patient samples, performed cDNA synthesis, and conducted gene expression analysis to identify differential expression patterns. I am well-versed in primer design, data analysis, and interpretation of gene expression results.
5. Can you share an example of a project or task you were assigned by a project leader that was outside your regular responsibilities? How did you approach and complete the task?
   • Answer: In a previous research position, I was assigned the task of optimizing a novel cell-based assay for drug screening. Although it was not part of my initial responsibilities, I approached the task with enthusiasm and a proactive mindset. I thoroughly researched the assay principles, reviewed relevant literature, and sought guidance from experienced colleagues. Through iterative experimentation and troubleshooting, I successfully optimized the assay conditions, resulting in improved sensitivity and reproducibility. This experience allowed me to showcase my adaptability, problem-solving skills, and commitment to project objectives.

Remember, these sample answers can serve as a reference point. It’s important to personalize your responses based on your own experiences and qualifications during the interview.