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DBT JRF Jobs

Dr. Reddy’s Institute of Life Sciences (DRILS) is a not for profit Research Institute with a mission to be the preferred research partner to the pharma and life sciences industry. DRILS focuses on original research and advancement of relevant skills, with emphasis on solution-oriented interdisciplinary research both for solving unmet needs as well as process innovation.

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Name of the Job: Junior Research Fellow

Project Title: “Molecular analysis of the functional role of ser/thr phosphatase PHLPP in lipid induced formation of foam cells, a hallmark of atherosclerosis”

Fellowship: Rs. 25,000 plus 30% HRA (total Rs. 32,500) per month for first two years and Rs. 28,000 plus 30% HRA (total Rs. 36,400) per month for 3 rd year

Duration: The duration of the fellowship is for three years. However, the performance of the candidate will be rigorously reviewed every 6 months and the fellowship will be renewed only upon satisfactory performance.

Responsibilities:

1) Literature search.
2) Design, plan and execute experiments under the supervision of the scientist.
3) Provide scientific support to the scientist in his research activities.
4) Book keeping and maintenance of stocks and consumables.

Scope of experimental work:

  • Generation and genotypic analysis of PHLPP-knockout zebrafish using CRISPR/Cas
    technology
  • Dissection of adult zebrafish, sectioning and slide preparation, and immunohistochemistry
  • Zebrafish blood collection and biochemical parameter testing
  • Staining and visualization of blood vessels and cells in adult and larval zebrafish
  • Routine cell culture, transfection of cells and analysis by western blotting, qPCR, ChIP,
    microscopy and various biochemical kits
  • Cloning and site directed mutagenesis
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Essential Qualifications:

A) Required:

1) Post-graduate degree in Biotechnology or any other related branch of Basic Sciences from a recognized university/institute with CSIR NET qualification.

OR

Graduate degree in a Professional course from a recognized university/institute with CSIR NET qualification

OR

Post-graduate degree in a Professional course such as MTech, MPharm, MVSc. etc
2) Candidate should demonstrate consistent academic record of minimum 60% aggregate in all qualifying examinations.
3) The candidate should be motivated and committed.

B) Experience: 1-2 years in the areas of Molecular Biology and Cell biology.

C) Preferable: Candidates with prior hands-on experience with zebrafish and cell culture are preferred. Experience with CRISPR and/or dissections and immunohistochemistry would be an additional advantage.

Project summary:

Atherosclerosis is a chronic inflammatory vessel disease occurring due to the accumulation of lipids in the arterial wall. Atherosclerosis is the principal cause of cardiovascular diseases
(CVD), the leading killer worldwide. Despite the management of lipids with current medications, there is a strong unmet medical need for the treatment of atherosclerosis necessitating development of newer and better therapies. Accumulation of lipid loaded foamy macrophages (foam cells) in arterial intima is the hallmark of the early atherosclerotic lesions. The main objective of the project is to explore the role of ser/thr phosphatase PHLPP in the formation of foam cells.

How to Apply:

Qualified and interested candidates may send their curriculum vitae by e-mail to [email protected] on or before 25th February 2019 with “DBT-PHLPP Project” as the subject line of the email. There will be two rounds of interview. Shortlisted candidates will be first interviewed through skype/telecon followed by a personal interview at Dr. Reddy’s Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad.

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Deadline – 25th February 2019

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