May 21, 2013
Phycobilins
Phycobilins are complex photoreceptor pigments – open-chain tetrapyrroles that are structurally related to mammalian bile pigments. Phytochromes are phycobilin-protein pigments involved in floral induction. There are two classes of phycobilins and they occur only in Cyanobacteria and Rhodophyta. The phycobilin component is similar to the porphyrins without a metallic atom.Water-soluble phycobilin pigments are found in the stroma of the chloroplast In at least two groups of algae, phycobiliproteins are aggregated in a highly ordered protein complex called a phycobilisome(PBS), making these phycobilins unique among photosynthetic pigments.
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Phycobilins are not only useful to the organisms which use them for soaking up light energy; they have also found use as research tools. Both phycocyanin and phycoerythrin fluoresce at a particular wavelength. That is, when they are exposed to strong light, they absorb the light energy, and release it by emitting light of a very narrow range of wavelengths. The light produced by this fluorescence is so distinctive and reliable, that phycobilins may be used as chemical "tags". The pigments are chemically bonded to antibodies, which are then put into a solution of cells. When the solution is sprayed as a stream of fine droplets past a laser and computer sensor, a machine can identify whether the cells in the droplets have been "tagged" by the antibodies. This has found extensive use in cancer research, for "tagging" tumor cells.
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