Experimental Conversions - Important for all Life Science Entrances as well Lab Work

Hi Friends,
We perform lot of experiments in laboratory and find it very confusing at the end to solve the calculation involved and to convert in the desired units it a tedious job. For e.g For a 1 picomolar solution how much amount of solute should be present in the given solvent. or convert microgram per mole to milligram per mole or viceversa. Its really a hectic job to do.So here are a few basic conversions which may be helpful and can make your work easy. In entrances too you will find these conversion units convinient to solve the problems.

Metric Prefixes

Prefix                            Symbol                    Factor
kilo                                   k                              10³
centi                                  c                              10^-2
milli                                   m                              10^-3
micro                                 µ                               10^-6
nano                                  n                               10^-9
pico                                   p                               10^-12
femto                                 f                                10^-15
atto                                   a                                 10^-18
zepto                                 z                                 10^-21

Spectrophotometric Conversions

1 A₂₆₀ unit of double-stranded DNA=50 µg/ml
1 A₂₆₀ unit of single-stranded DNA=33 µg/ml
1 A₂₆₀ unit of single-stranded RNA=40 µg/ml

Protein Molar Conversions

100 pmol of 100 kDa protein = 10 µg
100 pmol of 50 kDa protein = 5 µg
100 pmol of 10 kDa protein = 1 µg
100 pmol of 1 kDa protein = 100 ng

Protein/DNA Conversions

1 kb DNA =          333 amino acids   = 37 kDa protein
270 b DNA                                       = 10 kDa protein
810 b DNA                                       = 30 kDa protein
1.35 kb DNA