You are herePost Doctoral fellow Jobs at MRC Clinical Sciences, London
Post Doctoral fellow Jobs at MRC Clinical Sciences, London
Biochemistry, Molecular Biology & Structural Biology:
Analysis of the DNA Replication machinery
2 years fixed-term (with possibility for extension)
MRC Band 4 Salary £29,663 - £35,399 pa inclusive
London W12
Applications are invited for a postdoctoral research position within the DNA Replication group at the MRC Clinical Sciences Centre. We are looking for a very motivated biochemist that is interested to join our international team. The candidate will have the opportunity to join an ongoing very fast developing research project.
Recently we have characterized the assembly process of the ORC-Cdc6 complex on DNA (Speck et al. 2005 Nature Structural & Molecular; Speck and Stillman 2007 JBC; Speck et al 2008 PNAS) the first step in pre-RC formation. Now we have purified the MCM2-7 helicase and aim to characterise its helicase activity on its own and in context with other replication proteins. Further we want to analyse the influence of kinases on helicase activity. The activity of the MCM helicase will be tested in vitro and new mechanism of actions will be identified with the help of mutants in the helicase or accessory proteins. The postholder will work with other team members towards reconstitution of an active replication fork. In addition we want to identify the structure of the MCM2-7 complex alone and in the context of accessory proteins using electron microscopy. The methods include biochemical and biophysical approaches and structural work in an ongoing collaboration with a Cryo EM lab.
For further information visit: http://www.csc.mrc.ac.uk/Research/Groups/EDC/DNAReplication/ or
http://dnareplication.csc.mrc.ac.uk/
The successful candidate will have a Ph.D. degree in life sciences together with first-author publications and will be highly motivated, committed and have clear career goals. A strong background in biochemistry and protein purification is essential. Experience in helicase activity and protein- DNA interaction analysis would be beneficial.
The Clinical Science Centre, which is directly funded by the Medical Research Council, is a division of Imperial College. The CSC and Imperial College provide excellent core facilities and support structures. The DNA Replication Laboratory is part of the Epigenetics, Development and Cancer Section and offers interactions with groups studying cell cycle, cancer, nuclear organization and chromatin.
Last Date to Apply: 26th May 2009
