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International Training on Cryopreservation at NBPGR, Delhi


International Training Course In Vitro and Cryopreservation Techniques for Conservation of Plant Genetic Resources 9-21 November 2009, New Delhi, India.
The International Training Course is being organized by the NBPGR-Bioversity International Centre of Excellence. The National Bureau of Plant Genetic Resources (NBPGR) is one of the leading institutes under the Indian Council of Agricultural Research (ICAR), New Delhi, India for plant genetic resources (PGR) management. The 2009 training course is being co-organized by the Asia-Pacific Consortium on Agricultural Biotechnology (APCoAB), a programme of Asia-Pacific Association of Agricultural Research Institutions (APAARI). The course will be conducted from 9 to 21 November 2009 at NBPGR, Pusa Campus, New Delhi, India.

Course objectives
The course is designed for those currently involved in the development and use of in vitro and/or cryopreservation techniques for the medium to long-term conservation of germplasm of vegetatively propagated and non-orthodox seed species.
This course aims to:
• Improve skills of participants in using tissue culture techniques for conservation and management of PGR;
• Equip participants with essential knowledge necessary for developing and using cryopreservation techniques;
• Enhance the use of in vitro conservation and cryopreservation protocols for germplasm of crops relevant in their countries;
• Acquaint the participants with molecular techniques for PGR management.

Practical sessions
1. Preparation of culture media
2. Preparation of solutions/cryoprotectants for cryopreservation
3. Preparation/isolation of shoot tips for cryopreservation
4. Cryopreservation of shoot tips
5. Cryopreservation of seeds, zygotic embryos, embryonic axes, dormant buds and pollen
6. Cryobanking procedures
7. Molecular marker techniques- RAPD, ISSR, STMS, SCAR

Application
Applicants must be actively working on in vitro conservation and cryopreservation of PGR and preferably be experienced in using in vitro conservation, cryopreservation and molecular marker techniques in their own work.
The application form may be downloaded here. It should be completed and sent to:
The Director
National Bureau of Plant Genetic Resources
Pusa Campus, New Delhi-110012, India
Tel: +91-11-25843697 Fax: +91-11-25842495
Email: director@nbpgr.ernet.in
or
The South Asia Coordinator
Bioversity International, Sub-regional Office for
South Asia
NASC Complex
Pusa Campus, New Delhi 110012, India
Tel: +91-11-25849000/01 Fax: +91-11-25849002
Email: p.mathur@cgiar.org
Deadline for applications: 30 September 2009
Maximum number of participants: 15

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