June 20, 2013
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GATE 2010 Daily Quiz - Purify The Mixture of Proteins Today!
Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture based on differential partitioning between the mobile and stationary phases. Subtle differences in compounds partition coefficient results in differential retention on the stationary phase and thus separation.
Match up each of the mixtures (i-iii) listed below with the most appropriate separation method (a-e) and justify your choices:
Mixtures:
i) peptides Ser-Pro-Gly, Val-Leu-Phe and Glu-Asn-Arg
ii) peptides Ser-Thr-Gln, Asn-Trp-Leu-Ser-Pro and Asp-Leu-Lys-Glu-Arg-Phe-Ser-Gly
iii) two proteins of 20 kDa but with different ratios of Asp and Glu to His, Lys and Arg.
iv) a protein of 100 kDa that exists as a simple monomer and a polypeptide of 100 kDa that
forms a polymeric quarternary structure of > 2000 kDa.
v) a complex mixture of proteins of varied sizes and charges.
a) Gel filtration chromatography
b) SDS-Polyacrylamide gel electrophoresis
c) Ultracentrifugation
d) reversed phase chromatography
e) ion exchange chromatography
Choice 1
i - RPC
ii - IEC
iii - Ultracentrifugation
iv - GFC
v - SDS-PAGE
Choice II
i - IEC
ii - GFC
iii - RPC
iv - Ultracentrifugation
v - SDS-PAGE
Choice III
i - RPC
ii - GFC
iii - IEC
iv - Ultracentrifugation
v - SDS-PAGE
The Answer to yesterdays Enzyme Kinetics Problem is Choice I because
Specific activity = turnover number / molar mass
Rate of reaction = enzyme activity / reaction volume
Enzyme activity = specific activity x mass of enzyme present
Students who had provided correct answers are
AVINASH KUMAR
NIHARIKA MEHROTRA
shilpa
Dhananjay kharate
vishad gupta
GOKULA KRISHNAN
samrat sen
Congratulations!!
See Also